Susceptibility of Protein Synthesis to Neomycin in Neomycin-producing Streptomyces fradiae
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چکیده
منابع مشابه
The Production of Neomycin by Streptomyces Fradiae
Of basic importance to the study of antibiotic production by microorganisms is a chemically defined medium in which the effect of various nutrients, both organic and inorganic, can be studied with a minimum of interference from ill-defined substances. Such a medium is reported here for the production of neomycin by Streptomyces fradiae, together with studies on the influence of various nitrogen...
متن کاملRelationship between alkaline phosphatase and neomycin formation in Streptomyces fradiae.
Studies on phosphatase activity of Streptomyces fradiae 3535 grown in three different media indicate that neomycin formation varies directly with enzyme activity, sodium nitrate-maltose-mineral salts medium giving the highest yields of alkaline phosphatase and neomycin. S. fradiae contains more than one alkaline phosphatase and the phosphatase responsible for hydrolysis of neomycin phosphate ap...
متن کاملSemi-solid-state fermentation: a promising alternative for neomycin production by the actinomycete Streptomyces fradiae.
The production of neomycin by the actinomycete Streptomyces fradiae, under semi-solid-state fermentation conditions was the main subject of this study. Two supports (nylon sponge and orange peelings) were tested in order to determine the most suitable one for the production of neomycin by the above-mentioned microorganism. Nylon sponge led to the highest neomycin production, reaching a maximum ...
متن کاملOptimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation
Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the cr...
متن کاملIsolation and characterisation of an aminoglycoside phosphotransferase from neomycin-producing Micromonospora chalcea; comparison with that of Streptomyces fradiae and other producers of 4,6-disubstituted 2-deoxystreptamine antibiotics.
The gene (aphA-5c) encoding for aminoglycoside-O-phosphoryl-transferase (APH(3')-Vc) from Micromonospora chalcea 69-683 was cloned by expression in Streptomyces lividans; it showed strong nucleic and amino acid similarities with previously described streptomycetes aph genes. S1 mapping of the 5' region indicated that, as with several streptomycete genes, transcription apparently initiates at th...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1980
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-121-2-477